{
  "_meta": {
    "title": "Wastewater Epidemiology Detection Model",
    "author": "Brian Beaulieu",
    "date": "2026-07-19",
    "version": "1.0",
    "description": "Agent-legible specification for viral RNA detection in municipal wastewater systems",
    "source_article": "http://www.redrocknews.com/2026/07/18/measles-found-in-sedonas-wastewater/",
    "wikidata_entities": ["Q98894377", "Q79793"]
  },
  "detection_pipeline": {
    "stages": [
      {
        "stage_id": "sampling",
        "process": "composite_grab_24hr",
        "precision_target": "±2hr_window",
        "failure_mode": "flow_dilution_error",
        "sigma_contribution": 0.12
      },
      {
        "stage_id": "concentration",
        "process": "electropositive_filtration",
        "precision_target": "≥95%_recovery",
        "failure_mode": "membrane_clogging",
        "sigma_contribution": 0.08
      },
      {
        "stage_id": "extraction",
        "process": "magnetic_bead_binding",
        "precision_target": "Ct≤35",
        "failure_mode": "inhibitor_co_isolation",
        "sigma_contribution": 0.15
      },
      {
        "stage_id": "quantification",
        "process": "droplet_digital_pcr",
        "precision_target": "CV<5%",
        "failure_mode": "stochastic_partition_error",
        "sigma_contribution": 0.05
      }
    ],
    "total_uncertainty_budget": {
      "formula": "σ_total = √(Σσ²_i)",
      "computed_sigma": 0.219,
      "detection_threshold_multiplier": 3,
      "effective_threshold": 0.657
    }
  },
  "target_pathogen": {
    "name": "measles",
    "wikidata_qid": "Q79793",
    "classification": [
      "notifiable_disease",
      "viral_infectious_disease",
      "pandemic_and_epidemic_prone"
    ],
    "shedding_matrix": "fecal_respiratory",
    "environmental_stability_hours": 72
  },
  "deployment_spec": {
    "habitat_module_capacity": "500_residents_per_intake",
    "regional_hub_aggregation": "10_modules_per_hub",
    "alert_latency_target_days": 3,
    "baseline_sampling_frequency_hrs": 24
  },
  "validation_sources": [
    {
      "type": "news_event",
      "headline": "Measles found in Sedona's wastewater",
      "url": "http://www.redrocknews.com/2026/07/18/measles-found-in-sedonas-wastewater/",
      "date": "2026-07-18"
    },
    {
      "type": "knowledge_graph",
      "entity_slug": "wastewater-based-epidemiology",
      "wikidata_qid": "Q98894377",
      "url": "https://4ort.xyz/entity/wastewater-based-epidemiology"
    }
  ]
}